Amazing images from microscopes

The Nikon Small World web site has some stunning images and videos.

Image: Growing tip of a red algae by UNC's own Dr. Nat Prunet. Go Heels! See this and other images from UNC here.

Breaking the resolution limit of light microscopy

image credit: HHMI

Here's a photo of the original PALM microscope in the living room where it was developed, and a story about this and related technologies. The Wikipedia page on super-resolution microscopy discusses PALM and several other tricks for breaking the limit of resolution for light microscopy, and this article is a nice description of the challenges and multiple clever solutions to improving resolution beyond the usual limit.

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"...that’s where we learned about photoactivatable fluorescent proteins.... it became obvious to Harald and me that this was the missing link for the idea that I had pitched after I left Bell: we could isolate a few molecules at a time by activating limited subsets of photoactivatable proteins. It seemed so easy."

"...We were both unemployed, but Harald had some of his equipment from Bell [Labs]..."

-from Betzig's Nobel speech

Xiaowei Zhuang's lab developed a similar method, called STORM, around the same time as PALM was developed. See "STORM image gallery" at her lab web site for some super-resolution images. 

 

Struggling with your grades? Fear not!

Watch the 45 seconds between 1:45 and 2:30 to see how Chemistry Nobel Laureate Marty Chalfie (of Green Fluorescent Protein fame) did in Chemistry classes in college.

Louis Pasteur and his home

Louis Pasteur's old house is now a museum, currently under renovation through 2028. You can see it well documented here. Pasteur is buried in the basement, in an elaborate crypt (pictured here). 

Microns, nanometers, etc.

Powers of Ten, a classic Charles & Ray-Bernice Eames film on grasping the sizes of things from human scale out to the then-known universe, and back in to subatomic particles.

More images from Robert Hooke's Micrographia

Here's the whole book.

Hooke's book begins with an apology to the King of England, for, well, discovering things.

Hooke's methods for immobilizing some of the insects were creative:

...I gave it a Gill of Brandy, or Spirit of Wine, which after a while e'en knock'd him down dead drunk, so that he became moveless...

Hooke's engraving of a head of a hoverfly, from Micrographia

My favorite of van Leeuwenhoek's letters, with a surprise ending

"Concerning the Worms in Sheeps Livers, Gnats, and Animalcula in the Excrements of Frogs" (van Leeuwenhoek, 1700)

In this letter, he describes finding microscopic life in frog poop. I especially like the surprise ending in the last 3 paragraphs – starting, "On the fifth day the Frog had dung'd again...." on the bottom of page 517. Scientific papers never end like this.

Google on van Leeuwenhoek's 384th birthday

A popular book about cell biology

A great, vivid exploration of some fascinating topics in cell biology. See the "Read sample" button on the left side of this page to read parts of the book now. Also available as an audiobook.